LF 402 Metabolic

Highly sensitive and selective metabolism analysis for cell and tissue culture

LF 402 Metabolic || Principle of operation || Technical specifications || Applications

Applications

Technical documentation

  • Tumor diagnostics
  • Brain activity
  • Monitoring of organ models
  • Differentiation of tissue types
  • Basic research in medicine
  • Bioreactor monitoring (E.Coli, mammalian cells, insect cells)
  • LF402 Metabolic – Metabolism Detector

Literature

Hamann M, Richter A, Fink H, Rex A.
Altered nicotinamide adenine dinucleotide (NADH) fluorescence in dt sz mutant hamsters reflects differences in striatal metabolism between severe and mild dystonia.
J Neurosci Res. 2009 Feb 15; 87(3):776-83.
Pfeifer L, Grünwald I, Welker A, Stahn RM, Stein K, Rex A.
Fluorometric Characterization of Metabolism Activity of Ex Vivo Perfused Pig Hearts.
Biomed Tech (Berl). 2007; 52(2):193-199
Rex A, Fink F.
Applications of Laser-induced Fluorescence Spectroscopy for the Determination of NADH in Experimental Neuroscience
Laser Phys Lett 2006; 3(9): 452-459
Rex A, Fink H.
Effects of 8-OH-DPAT on Hippocampal NADH Fluorescence In Vivo in Anaesthetized Rats
Journal of Neuroscience Research 2006; 83: 551-556
Rex A, Hentschke M-P, Fink H.
Bioavailability of Reduced Nicotinamide-adenin-dinucleotide (NADH) in the Central Nervous System of the Anaesthetized Rat Measured by Laser-Induced Fluorescence Spectroscopy
Pharmacology & Toxicology 2002; 90: 220-225
Büchner M, Huber R, Sturchler-Pierrat C, Staufenbiel M, Riepe MW.
Impaired Hypoxic Tolerance and Altered Protein Binding of NADH in Presymptomatic App23 Transgenic Mice
Neuroscience 2002; 114(2): 285-289
Plettenberg HKW, Hoffmann M.
Applications of Autofluorescence for Characterisation of Biological Systems (Biomonitoring)
Biomed Tech (Berl) 2002; 47 Suppl 1 Pt2: 596-597
Rex A, Pfeifer L, Fink H.
Determination of NADH in Frozen Rat Brain Sections by Laser-Induced Fluorescence
Biol Chem, Vol 2001; 382: 1727-1732
Schuchmann S, Kovacs R, Kann O, Heinemann U, Buchheim K.
Monitoring NAD(P)H Autofluorescence to Assess Mitochondrial Metabolic Functions in Rat Hippocampal-Entorhinal Cortex Slices
Brain Research Protocols 2001; 7: 267-276
Huber R, Büchner M, Li H, Schlieter M, Sperfeld AD, Riepe M.
Protein Binding of NADH on Chemical Preconditioning
Jour Neurochem 2000; 75(1): 329-335
Rex A, Pfeifer L, Fink F, Fink H.
Cortical NADH during Pharmacological Manipulations of the Respiratory Chain and Spreading Depression In Vivo
Jour Neurosc Res 1999; 57: 359-370
Maerz HK, Buchholz R, Emmrich F, Pfeifer L, Marx U.
Development of a Pharmacological Test System in a Miniaturized Hollow Fiber Bioreactor
in: "Microreaction Technology"; Editor: W Ehrfeld

Springer Berlin, Heidelberg, New York (1998)
Maerz HK, Buchholz R, Emmrich F, Fink F, Geddes CL, Pfeifer L, Raabe F, Scheper T, Ulrich E, Marx U.
Applicating Fiber Optical Methods for Toxicological Testing in Vitro
Proceedings of Systems and Technologies for Clinical Diagnostics and Drug Discovery II
Editors: Cohn, J C, Owicki, J C

SPIE Vol 3603, 228 - 239, (1998)
Riepe MW, Schmalzigaug K, Fink F, Oexle K, Ludolph AC
NADH in the Pyramidal Cell Layer of Hippocampal Regions CA1 and CA3 upon Selective Inhibition and Uncoupling of Oxidative Phosphorylation
Brain Res 1996; 710: 21-27
Maerz HK, Buchholz R, Emmrich F, Fink F, Geddes CL, Pfeifer L, Raabe F, Marx U.
Monitoring Tissue Metabolism via Time-Resolved Laser Fluorescence
Proceedings of Advances in Fluorescence Sensing Technology IV
Editors: Lakowicz JR, Soper SA, Thompson RB

SPIE Vol 3602, 24 - 35, (1998)
Maerz H et al.
Development of a Pharmacological Test System in a Miniaturized Hollow Fiber Bioreactor
Proceedings of the 1st International Conference on Microreaction Technology, 23-25 Feb 97 in Frankfurt/M, Germany, Springer (1998)
Pfeifer L, Paul R, Yalcin E, Marx U, König F, Fink F.
A time-gated spectrometer using optical fibres for detecting fluorescent biomolecules in cells and tissue
7th Int Conf on In Vivo Methods - Monitoring molecules in Neuroscience; St.Cruz de Tenerife, Spain, S 42-43, (1996)
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