cAMP Detection Assay
for pharmaceutical and biotech research
Novel options for high-throughput laboratories in cAMP screening
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SBS2008 Tutorial (PDF) (1.5MB)
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Your benefits
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Technical advantages
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| Suppression of compound/background fluorescence | |
| High signal-to-noise ratio | |
| High dynamic range | |
| Good linearity | |
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| One-step reagent addition saves labor and time | |
| Color-coded reagents | |
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| Insensitive to divalent cations (Mg2+, Mn2+) and chelators (EDTA, EGTA) | |
| Insensitive to high ATP concentrations | |
| Cell-based assay, but also works with preparations of membranes | |
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| Signal can be read after up to 24 h (room temperature) | |
| Working solution is stable at 4 °C for at least 2 months | |
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| Theoretical and hands-on assistance comprising solutions to technical questions and adaptation of the test to the individual requirements. | |
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Assay description
on the detection of fluorescence resonance energy transfer (FRET)
thus providing a homogeneous assay format.
Read-out requirements
| Excitation | 456 nm, nanosecond light pulses |
| Emission donor channel | 620..630 nm, Integration 3500 ns, Delay 1500 ns |
| Emission acceptor channel | 700..730 nm, Integration 300 ns, Delay 200 ns |
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Experimental data
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Kit design
5,000 data points and
44,000 data points.
One data point is defined as the addition of 10 µl of working solution
(entire volume per well: 30 µl).
Kit content
| Description | Bottle/Cap Color | Shipping/Storage Condition |
| 1. Ru-cAMP solution | cup with red lid in alu container | -20°C/-20°C |
| 2. anti cAMP-IgG AlexaFluor 700 solution | cup with blue lid in alu container | -20°C/-20°C |
| 3. 3x lysis buffer | polyethylene bottle | -20°C/-20°C |
Not for use in diagnostic procedures.
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Assay protocol
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| Simply add the two assay components to the 3x lysis buffer to prepare the cAMPNano- TRF working solution.  |
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| For setting up the screening assay simply add the prepared cAMP-Nano-TRF working solution to your test vials.  |
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| cAMP-Nano-TRF working solution is used by adding one part of cAMP working solution (10 µl) to two parts of HTS reaction mix (20 µl). Downscaling is possible provided this ratio is retained. |
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Trademarks
CAMP-NANO-TRF is a trademark of Roche.
Other brands or product names are trademarks of their respective holders.
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Disclaimer
between Molecular Probes, Inc., a wholly owned subsidiary of Invitrogen
Corporation, and Roche Diagnostics GmbH and the manufacture, use, sale or
import of this component and conjugates and combinations thereof is subject
to one or more pending US applications and corresponding international
equivalents, owned by Molecular Probes, Inc. The purchase of this product
conveys to the buyer the non-transferable right to use this product for research
use only for performing TR-FRET assays (including HTS assays), including
contract research services for performing TR-FRET assays (including HTS
assays). The buyer cannot sell or otherwise transfer this product to a third party
or otherwise use this product for non-research use. For information on
purchasing a license to this AlexaFluor 700 dye for purposes other than as set
forth above, contact
Molecular Probes, Inc., Business Development,
29851, Willow Creek Road, Eugene, OR 97402-9132.
Phone: +1 (541) 465-8300. Fax: +1 (541) 335-0504.
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| cAMP Assay |
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